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In this work, we have taken a donor–acceptor–donor (D–A–D) fluorophore ( II-EDOT-TPA ) and encapsulated it using a linear dendritic block copolymer (LDBC). In parallel, a polyethylene glycol derivative ( PEG-II-EDOT-TPA ) was synthesized. The self-assembly and colloidal properties of both nanoaggregates were comparatively assessed. Photophysical and morphological characterization of the LDBC encapsulated II-EDOT-TPA and PEG-II-EDOT-TPA nanoaggregates was performed, which showed the photophysical and morphological properties differed greatly when comparing the two. Both nanoaggregate types were incubated with HEK-293 cells in order to measure cell viability and perform confocal fluorescence microscopy. Minimal cytotoxicity values (<20%) were seen with the two nanoaggregate forms, while both types of nanoaggregates were found to accumulate into the lysosomes of the HEK-293 cells. This work provides fascinating insights into NIR fluorophore design and methods to effectively alter the photophysical and morphological properties of the nanoaggregates for bio-imaging purposes. 

Shortwave infrared (SWIR) emission has great potential for deep-tissue in vivo biological imaging with high resolution. In this article, the synthesis and characterization of two new xanthene-based RosIndolizine dyes coded Ph RosIndz and tol RosIndz is presented. The dyes are characterized via femtosecond transient absorption spectroscopy as well as steady-state absorption and emission spectroscopies. The emission of these dyes is shown in the SWIR region with peak emission at 1097 nm. Tol RosIndz was encapsulated with an amphiphilic linear dendritic block co-polymer (LDBC) coded 10-PhPCL-G3 with high uptake yield. Further, cellular toxicity was examined in vitro using HEK (human embryonic kidney) cells where a >90% cell viability was observed at practical concentrations of the encapsulated dye which indicates low toxicity and reasonable biocompatibility. 

Summary High performance computing (HPC) has led to remarkable advances in science and engineering and has become an indispensable tool for research. Unfortunately, HPC use and adoption by many researchers is often hindered by the complex way these resources are accessed. Indeed, while the web has become the dominant access mechanism for remote computing services in virtually every computing area, HPC is a notable exception. Open OnDemand is an open source project negating this trend by providing web‐based access to HPC resources (https://openondemand.org). This article describes the challenges to adoption and other lessons learned over the 3‐year project that may be relevant to other science gateway projects. We end with a description of future plans the project team has during the Open OnDemand 2.0 project including specific developments in machine learning and GPU monitoring. 

Abstract Here, we demonstrate the applicability of self‐assembling linear‐dendritic block copolymers (LDBCs) and their nanoaggregates possessing varied surfaces as therapeutic nanocarriers. These LDBCs are comprised of a hydrophobic, linear polyester chemically coupled to a hydrophilic dendron polyamidoamine (PAMAM)—the latter of which acts as the surface of the self‐assembled nanoaggregate in aqueous media. To better understand how surface charge density affects the overall operability of these nanomaterials, we modified the nanoaggregate surface to yield cationic (NH₃⁺), neutral (OH), and anionic (COO⁻) surfaces. The effect of these modifications on the physicochemical properties (i.e., size, morphology, and surface charge density), colloidal stability, and cellular uptake mechanism of the polymeric nanocarrier were investigated. This comparative study demonstrates the viability of nanoaggregates formed from PDLLA‐PAMAM LDBCs to serve as nanocarriers for applications in drug delivery.